Optimization of Microalgae Biomass Yield using Chlorella Sp. and Spirulina Sp. Cultivation in secondary treated wastewater effluent

Abstract Conventional method for domestic wastewater treatment could be inefficient due to active organic loadings and high population. Thus, the treated still contains nutrient of nitrogen, phosphorus, ammonia that can lead eutrophication threaten biodiversity in long run. This study is aimed concentrate on capability microalgae, Chlorella sp. Spirulina cultivated with secondary effluent nutrients removal biomass yield. The sample was collected analysed characterization total nitrogen (TN = 2.78 mg/L) phosphorus (TP 2.13 using standard by APHA. optimization best dilution microalgae ratio were run Research Surface Methodology (RSM). optimum results found which 100% mixed 50% 70% dilution. highest obtained from yield 0.22 g/L. While NPK scanning electron microscopy energy dispersive spectroscopy (SEM-EDS) 0.6 wt% 0.3 (P) potassium (K) respectively. Subsequently, potential traits as a biofertilizer positively observed based these responses. shows able grow have ability become content.